H7 Virus Avian Influenza Rapid Test Kit Cassette Format Multiple Specimen

Avian influenza virus H7 antigen rapid test card ,4mm cassette rapidly and easily, gold colloidal method

Intended Use

Avian influenza hemagglutinin according to their outer membrane (H) and neuraminidase (N) protein antigen is divided into a plurality of different and different subtypes. H7 avian influenza virus antigen rapid test card is specifically designed to detect H7 subtypes of the virus detection reagent.

The AIV H7 Ag test strip is based on fast immuno-chromatography technique to detect AIV H7 Ag in birds trachea or cloaca (or feces). After adding sample, the sample move along with the colloidal gold labeling H7-AIV antibody, is there is H7-AIV antigen in the sample, it combines with antibody on T line and show wine-red color; if there is no H7-AIV antigen, then no color reaction.

Components

Test Procedure

1) Use Swab to collect sample from cloaca (recommended) or trachea, the swab should be inserted into cloaca or trachea, wipe and rotate in inner well repeatedly. To young birds, it can use dropper to collect fresh feces directly.

2) Insert the swab into the tube with sample buffer immediately, rotate the swab strongly on tube well for at least 10 times and mix the solution evenly, making the sample been dissloved in sample buffer fully. Press the swab on tube well above the liquid level, making the liquid been pressed out fully, discard swab.

3) If the particles are big in feces, it may be static for 1-2 min, making bigger particles precipitate.

4) Take out test card from sealed bag. Put it on flat surface, use dropper to draw upper-layer bright liquid, drop 5 drops slowly, one by one, to the sample hole with “S” mark.

5) Put the test card at room temperature for 10-15min, read the result, the result is invalid after 15 minutes.

INTERPRETATION OF RESULTS

Positive: control line and test line are both seen wine red, the more the antibody exist, the thicker the color appear;

2) Weak positive: control line and test line are both seen wine red, but the color of test line is very light;

3) Negative: only control line is seen wine red;

4)　Invalidation: control line isn’t seen wine red.

QUALITY CONTROL

A procedural control is included in the test. A line appearing in the control region

(C) is considered an internal procedural control. It confirms sufficient specimen volume, adequate membrane wicking and correct procedural technique.

Control standards are not supplied with this kit. However, it is recommended that positive and negative controls be tested as good laboratory practice to confirm the test procedure and to verify proper test performance.

Note

1) Do not use product with broken Aluminum foil bag package. Use test card in 1 hour after taking out.

2) The collected sample should be tested immediately, avoid long time storage.

3) The sample collection directly affect the accuracy of testing, so ensure collect sample correctly and fully.

4) Collect feces sample too much will affect the normal surge of liquid, and lead to wrong result (black area appear), and too little sample may lead to false negative result, so collect sample as following picture.

5) Avoid bubble when adding sample to test card.

6) The used test card should be handled as local pollution properly.

7) Do not use test card out of expiry date.

8) Deal with the used test card and sample as waste according to local regulation strictly.

Frequently Asked Questions

1) Limitation of the product

This kit is a qualitative screening reagent, can detect AIV antigen accurately, but if sample is not enough or concentration of antigen is lower than sensitivity, it may appear negative result. A clear clinical diagnosis should be made after taking into account all clinical and laboratory phenomenon.

2) The sensitivity and specificity of the product.

The limit value to detect AIV H7 Ag is 0.13 HAU, reaction with H1N1 / H2N2 / H3N2 / H5N1 / H9N2 strains have a negative representative. it has no cross-reaction with similar symptoms to those of fowl cholera, newcastle disease, infectious bronchitis

Established in 2009, Orient New Life Medical Co., Ltd., is specialized in high-tech biological diagnostic test development, research and production, as well as high-tech medical device development, research and production. New Life has built up two production base, which produce biological diagnostic test and endoscopic medical device respectively. Our products ranged in the following specialties: drug of abuse tests, fertility tests, infectious diseases tests, sexual transmitted diseases tests, tumor markers tests, cardiac markers tests, veterinary tests, and surgical stapler series.

NEW LIFE have 3 wholly-owned and joint stock subsidiary companies, including Orient New Life Medical Co., Ltd., Orient Gene Biotech Co., Ltd., Qianjing Medical Equipment Co., Ltd.

Company's core technical team with more than 35 employees，3 doctors and 25 masters. They have many years of famous universities in the United States and pharmaceutical companies engaged in the project research and development, production and marketing, business management experience.Company owns hundreds of grade purification workshop and all level of quality checkout room, equipped with various production and testing equipment.