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Aflatoxin M1 ELISA kit for Fresh Milk Dairy Products 96Wells/Kit ISO9001
1. Mycotoxin ELISA Testing Kits Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Aflatoxins M1. The coupling antigen is pre-coated on the micro-well stripes. The Aflatoxins M1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Aflatoxins M1 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Aflatoxins M1 in the sample. This value is compared to the standard curve and the Aflatoxins M1 residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.03 ppb
Incubator temperature: 25℃
Incubator time: 30min~15min
Detection limit:
Milk | 0.1ppb |
Milk powder | 0.3ppb |
Yogurt | 0.3ppb |
Cross-reaction rate:
Aflatoxins M1 | 100% |
Aflatoxin B1 | 12.9% |
Aflatoxin B2 | 1.4% |
Aflatoxin G1 | 1.9% |
Aflatoxin G2 | 0.3% |
Recovery rate:
Milk | 90±25% |
Milk powder | 100±30% |
Yogurt | 100±30% |
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1 mL each) | 0ppb | 0.03ppb |
0.09ppb | 0.27ppb | ||
0.81ppb | 2.43ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 40ml | white cap |
9 | 10× concentrated redissolving solution | 50ml | transparent cap |
4. Sample pretreatment steps and detection and analysis steps
See product manual for details.
5. Storage conditions and shelf life
The kit should be stored at 2-8°C and should not be frozen.
Valid for 12 months.
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific
needs and specific quantities.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so
far all the production process, we have standard rules, we comply
with the relevant behavior and laws of the government.
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service.
If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
Q5: What is your minimum order quantity?
A5: 1Kit.
Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by
air and ground.Please confirm with us before placing an order.