Agarose NHS Magnetic Beads Preactivated N - Hydroxy Succinimide Nanoparticle
Brand Name:BeaverBeads
Certification:COA
Model Number:70705-50
Minimum Order Quantity:1 mL / bottle
Delivery Time:5-8 days
Payment Terms:T/T, L/C
Contact Now
Add to Cart
Verified Supplier
Location:
Suzhou China
Address:
NE36 Nanopolis Suzhou, 99 Jinji Lake Avenue, Suzhou Industrial Park
Supplier`s last login times:
within 17 hours
Product Details
Company Profile
Product Details
Agarose NHS Magbeads Preactivated N - Hydroxy Succinimide Nanoparticle 10 - 30 μm, 20% (v/v)
1, Description:
The NHS magnetic beads are superparamagnetic beads with activated
groups of N_Hydroxysuccinimide on the surface, which can react with
primary amines on desired ligands and bind them covalently with
amide linkage to the beads. The ligand can be various molecules
including antigen, antibody and other proteins. Compared with the
traditional carboxyl, amino magnetic beads, the NHS group of
magnetic beads don’t need to activate EDC/NHS or Glutaraldehyde,
simply dissolving the amino-containing bio-ligand in the coupling
buffer, at room temperature, mixing with proteins and the NHS
magnetic beads for only 1-2 hours, the bio-ligand can be coupled to
the magnetic beads. The advantages include easy operation, mild
coupling conditions and coupling fast and efficient. The magnetic
bead coupling process must be carried out in a buffer solvent free
of any amino. This method can be operated manually or
automatically.
The biological ligand can be covalently coupled to the magnetic
beads. It has the advantages of simple operation, mild coupling
condition and quick and efficient coupling of biological ligands.
The difference between BeaverBeads ™ Mag NHS and BeaverBeads ™
Magrose NHS is that Mag NHS is a polymer-based solid microsphere
with a particle size of 2 μm and is mainly used for analytical
areas (eg in vitro diagnostics, microbiological testing,
immunoprecipitation, immunization Precipitation, etc.); and Magrose
NHS is a natural hydrophilic polymer agarose as the substrate of
porous microspheres, particle size of 30 ~ 100μm, which has good
biocompatibility, a larger surface area and biological Based
coupling, mainly used for separation and purification of the field
(such as protein separation and purification, antibody isolation
and purification).
2, Introduction:
| Product Name | Agarose NHS Magbeads Preactivated |
| Mean particle size | 10-30 μm |
| Magnetic core | Fe3O4 |
| Shell | Agarose |
| Magnetic | superparamagnetism |
| Ligand content | ~50 μM/mL gel |
| Binding ability | 20~30 mg rabbit IgG/mL beads |
| Preservation fluid | 20% (v/v) ethanol solution |
| Operation | Automate/manual |
| * water average particle size, Malvern Nano determination | |
3, FEATURES:
1. Simple, without activation, can be directly covalently coupled with the biological ligand;
2. Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;
3. Fast, 1 ~ 2h to complete the bio-ligand coupling;
4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;
5. Stable, the formation of a stable amide bond, to prevent the ligand off.
6. Good biocompatibility, reduced nonspecific adsorption.
Agarose NHS Magnetic Beads Preactivated N - Hydroxy Succinimide Nanoparticle
Inquiry Cart
0