Effective Dibutyl Phthalate (DBP) ELISA Test Kit High Sensitivity (0.05 Ppb)
Brand Name:REAGEN
Model Number:RNC95008
Minimum Order Quantity:5 kits
Delivery Time:5-7 days
Payment Terms:T/T
Place of Origin:USA
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Dibutyl phthalate (DBP) ELISA Test Kit
Product Description
REAGEN™Dibutyl phthalate (DBP) is a kind of plasticizer, the DBP ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Dibutyl phthalate in water, juice, vinegar, wine and sauce.
The unique features of the kit are:
1. High recovery (70-130%), Rapid and cost-effective extraction methods for various samples.
2. High sensitivity (0.05 ppm)
3. High reproducibility.
4. A quick ELISA assay (less than 1.5 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (µg/g or ppm) |
| Water | 0.08 |
| Juice/vinegar/wine / sauce | 0.02 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Dibutyl phthalate | 100 |
| Butyl benzyl phthalate | 11.2 |
| Diisobutyl phthalate | 30.5 |
| Dioctyl phthalate | <0.1 |
| Diisononyl phthalate | <0.1 |
| Di-n-octyl-phthalate | <0.1 |
| Diisodecyl phthalate | <0.1 |
| P-hydroxybenzoic acid esters | <0.6 |
| Benzoate | <0.6 |
Effective Dibutyl Phthalate (DBP) ELISA Test Kit High Sensitivity (0.05 Ppb)
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