β-lactamase ELISA Kit , competitive enzyme immunoassay , detect beta-lactam containing antibiotics
Brand Name:REAGEN
Model Number:RND99082
Minimum Order Quantity:5 kits
Delivery Time:5-7 days
Payment Terms:T/T
Place of Origin:USA
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Product Details
β-lactamase ELISA Kit
Purpose
This kit allows for the determination of β-lactamase activity in milk.
Principle of the assay
The kit assay β-lactamase level in the sample,use Purified β-lactamase antibody to coat microtiter plate wells, make solid-phase antibody, then add β-lactamase to wells, Combined β-lactamase antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of β-lactamase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
Reqired Materials Not Provided With the Kit
- Microtiter plate reader (450 nm)
- Incubator
- Tissue Mixer (e.g. Omni TissueMaster Homogenizer)
- Vortex mixer (e.g. Gneie Vortex mixer from VWR)
- 10, 20, 100 and 1000 mL pipettes
- Multi-channel pipette: 50-300 mL (Optional)
- n-Hexane
- Distilled deionized water
- Disposable plastic reservoirs
- Paper towels
- Centrifuge
β-lactamase ELISA Kit , competitive enzyme immunoassay , detect beta-lactam containing antibiotics
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