Nocardia Seriolae Nucleic Acid Test Kit Biokey Multiplex Real Time PCR Kit

Brand Name:Biokey
Certification:ISO 13485
Model Number:BIK-Q-W028
Minimum Order Quantity:We can produce liquid and lyophilized kits
Delivery Time:Depending on order quantity
Payment Terms:L/C, T/T, Western Union
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Location: Guangzhou
Address: Add:No.2, Ruitai Rd, Guangzhou Hi-Tech Industrial Development Zone, 510670, CHINA
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Product Details

Biokey Super Sensitive Multiplex Real Time PCR Kit Nocardia seriolae Nucleic Acid Detection Kit


Intended use:


The kit is used for the epidemiological analysis of Nocardia seriolae in fish viscera tissue samples


Principle:

In this kit, the specific fragment of N.seriolae is used to design the primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclide activity of Taq enzyme cuts the 5 '-terminal fluorophore from the probe and makes it free in the reaction system. Therefore, the quenched 3 '-end group was separated and the fluorescence was emitted. The fluorescence PCR instrument was used to detect Nocardia seriolae in a completely closed reaction system

Main components:

ComponentSpecification
PCR reaction liquid24 tubes
Positive control1 tube
Negative control1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:

1. Sample type

Gills, spleen, kidney and other pathological tissues.

2. Sample collection and transportation

Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

PCR amplification and fluorescence detection (PCR detection area):

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:


StepCycle numberTemperatureTime
1194℃3min
24594℃10s
60℃20s collect fluorescent

FAM was selected as the detection channel.


Quality control:

  1. Negative control: No obvious amplification curve of FAM detection channel;
  2. Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;
  3. The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

The results of interpretation

ResultsCt Value
PositiveCt≤38
NegativeNo Ct value or Ct value≥45
Gray area38<Ct<45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


Product performance index:


1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 10copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

4. Linear, linear correlation coefficient | r | > 0.980.


Product picture:


Operation details please check from IFU!

China Nocardia Seriolae Nucleic Acid Test Kit Biokey Multiplex Real Time PCR Kit supplier

Nocardia Seriolae Nucleic Acid Test Kit Biokey Multiplex Real Time PCR Kit

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