RT PCR Norovirus Detection Kit Lyophilized Rotavirus Test Fluorescence Probe

Brand Name:Biokey
Certification:ISO 13485,CE
Model Number:BIK-QL-H007 / BIK-QL-H007S
Minimum Order Quantity:We can produce liquid and lyophilized kits
Delivery Time:Depending on order quantity
Payment Terms:L/C, T/T, Western Union
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Location: Guangzhou
Address: Add:No.2, Ruitai Rd, Guangzhou Hi-Tech Industrial Development Zone, 510670, CHINA
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Product Details

Multiplex RT-PCR four enteroviruses Fluorescence probing PCR test kit ( Lyophilized )


Intended use:

This kit is used for in vitro qualitative detection of Norovirus, Rotavirus, Adenovirus and Astrovirus nucleic acid RNA. It can be used for clinical auxiliary diagnosis of the etiological identification of suspected infected persons


Test Principle:


This kit uses fluorescent RT-PCR technology (TaqMan probe) to rapidly and accurately qualitatively detect the conserved regions of Norovirus/Rotavirus/Adenovirus/Astrovirus On the instrument, the real-time fluorescence quantitative PCR detection technology is used to realize the rapid detection of Norovirus/Rotavirus/Adenovirus/Astrovirus through the change of fluorescent signal; the detection system contains a positive internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.


Product components:


The product components as following:


1. BIK-QL-H007:

No.ComponentsAmount
1PCR Enzyme Mix (Lyophilized)96 tests/bottle
2

Primer-Probe Mix 1 (Norovirus and

Rotavirus)

50 μL/vial
3

Primer-Probe Mix 2 (Adenovirus

and Astrovirus)

50 μL/vial
4Enzyme Mix Buffer (5×)400 μL/vial
5Positive Control100 μL/tube
6Negative Control100 μL/tube
7RNase-free water1mL/tube
8Paraffin oil1.5mL/tube*2

2. BIK-QL-H007S:

No.ComponentsAmount
11 (Norovirus and Rotavirus Mix (Lyophilized))24 tests/kit
22 (Adenovirus and Astrovirus Mix (Lyophilized))24 tests/kit
3Positive Control100 μL/tube
4Negative Control100 μL/tube
5RNase-free water1mL/tube
6Paraffin oil1.5mL/tube

Data anlysis:


The following sample results are possible:

Ct valueResult Analysis
1#No CtNegative
2#≤38Positive
3#38~40Re-test; if it is still 38~40, report as 2#

Interpretation of test results:


(1)Norovirus and Rotavirus reaction:

FAM

(Norovirus)

VIC/HEX

(Rotavirus)

ROX

(IC)

Interpretation test results
1

-


-


-

Result invalid, test again
2+-+/-Norovirus(+)
3-++/-Rotavirus(+)
4+++/-Norovirus and Rotavirus(+)
5--+Negative(-)

(2)Adenovirus and Astrovirus reaction:

FAM

(Adenovirus)

VIC/HEX

(Astrovirus)

ROX

(IC)

Interpretation test results
1

-


-


-

Result invalid, test again
2+-+/-Adenovirus(+)
3-++/-Astrovirus(+)
4+++/-Adenovirus and Astrovirus(+)
5--+Negative(-)

NOTE: ‘+ ’ is positive, ‘- ’ is negative.


Operation details please check from IFU

China RT PCR Norovirus Detection Kit Lyophilized Rotavirus Test Fluorescence Probe supplier

RT PCR Norovirus Detection Kit Lyophilized Rotavirus Test Fluorescence Probe

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