High Efficiency Magnetic Bead DNA Extraction Kits For PCR Applications

High Efficiency Magnetic Bead DNA Extraction Kits For PCR Applications

Fast and High Efficiency Magnetic Bead DNA Extraction Kit Purification Collection Kit for PCR Applications

product description:

HUACHENYANG DNA Extraction Kit provides a fast and efficient magnetic bead method to purify and extract DNA (including genomic, mitochondrial and viral DNA) from preserved tissues, saliva, body fluids, as well as from oral cavity, cervix, skin cells, bacterial cells, etc. Biological samples can be stored at room temperature for up to 30 days in our proprietary storage buffer before processing without significant loss of DNA yield or quality (more than 30 days if stored frozen) No phenol/ Chloroform extraction or alcohol precipitation yields high-quality genomic DNA within 15 minutes, with an average DNA yield of 8 μg per buccal swab. Purified DNA, approximately 20-30 kb, suitable for downstream applications such as PCR or other enzymatic reactions

Features:

Using silicon magnetic beads and buffer system with unique separation function, it can quickly, highly sensitively and efficiently extract high-purity nucleic acid from samples, and the extracted and purified nucleic acid can be used for PCR, RT-PCR, digestion, reverse transcription, and Southern Various common downstream experiments such as blot, applicable specimen types: whole blood, tissue homogenate, swab, serum, plasma, bronchoalveolar lavage fluid, etc. Applicable extraction instruments: most of the automatic nucleic acid extraction instruments on the market

benefit:

1. Magnetic bead-based technology isolates genomic DNA without the need for hazardous chemicals, centrifugation or vacuum manifolds, phenol and ethanol precipitation.

2. Fast and efficient purification of genomic DNA from human buccal swabs in less than 15 minutes after sample preparation and lysis.

3. Simple cleavage with proteinase K without any mechanical cleavage.

4. Minimal RNA contamination.

5. Purified genomic DNA exhibits improved downstream performance in applications such as PCR.

6. A kit is included for automated processing of large numbers of samples in 96-well plates using a liquid handling robot.

Product Instructions:

1. Before using nucleic acid extraction reagents

①. Transfer proteinase k solvent to lyophilized powder containing proteinase k and mix well.
②Add 18ml and 42ml of absolute ethanol to CY3 and CY4 of CY-F006-10 (50preps-cells) and CY-F006-20 (50preps-saliva), stir well.
③. Add 36ml and 84ml of absolute ethanol to CY3 and CY4 of models CY-F006-11 (100preps-cells) and CY-F006-21 (100preps-saliva) and mix well.

2. Swab extraction steps:

① Dry swab collection, add 0.6ml CY1 solution and 10ul proteinase K, mix well, and incubate in a 65°C air incubator for 30 minutes (or wet collection: centrifuge the sample centrifuge tube containing the swab and preservation solution to 12000 Transfer for 1 minute, keep the precipitate, remove the supernatant, add 0.6ml of CY1 solution, 10ul of proteinase K, mix well, and incubate in an air incubator at 65 degrees Celsius for 30 minutes).
②. Remove the swab and centrifuge at 12000rpm for 1 minute.
③. Remove all supernatants into new centrifuge tubes and perform experiments.
④. Add 0.25ml of CY2 solution, 10ul of magnetic beads* (shake well before use), mix for 12min, put it on a magnetic stand for 30s, and blot dry.
⑤ Add 0.6ml of CY3 solution, stir for 3min, put it on a magnetic stand for 30s, and absorb the liquid.
⑥. Add 0.6ml of CY4 solution, mix for 3min, put it on a magnetic stand for 30s, and dry the liquid
⑦, repeat steps ②⑥
⑧ Dry at room temperature for 10-20min, add 50ul CY5 liquid for elution, mix well, place it on a magnetic stand for 30s, and then transfer the liquid to a new centrifuge tube
⑨, measure the outer diameter

3. Saliva Extraction Step

① Centrifuge saliva plus preservation solution at 12000rpm for 1min
② Retain the precipitate and remove the supernatant
③. Add 0.6ml of CY1 solution and 10ul of proteinase k, stir evenly, and incubate in an air incubator at 65 degrees Celsius for 30 minutes.
④ Centrifuge at 12000rpm for 1min, take out all the supernatant into a new centrifuge tube, add 10ul of magnetic beads and 0.25ml of CY2, mix for 12min, and place it on a magnetic stand for 30s to absorb the liquid.
⑤ Add 0.6ml of CY3 solution, stir for 3min, put it on a magnetic stand for 30s, and absorb the liquid.
⑥. Add 0.6 ml of CY4 solution, mix for 3 min, place it on a magnetic stand for 30 s, and absorb the liquid.
⑦, repeat step ⑥
⑧ Dry at room temperature for 10-20 minutes, add 50ul CY5 liquid for elution, mix well, put it on a magnetic stand for 30s, and then transfer the liquid to a new centrifuge tube.
⑨, measure the outer diameter

Note: To remove RNA, prepare RNaseA 10mg/ml: solvent (10mM sodium acetate: pH 5.0), boil for 15min, adjust pH 7.5 with Tris-HCl, and store at -20 degrees Celsius.

Precaution:

1. This product is only used for in vitro diagnosis.

2. The storage environment and extraction steps should be carried out in strict accordance with the instructions in the instruction manual.

3. If it is found that the amount is too small during the extraction process, the sample size can be appropriately increased or the number of extractions can be increased.

4. The extracted DNA must be fresh and tested in time.

Note: This transfer medium is intended for in vitro diagnostics and is not intended for internal or external use in humans or animals. If swallowed, it may cause a serious accident; it is irritating to eyes and skin. If splashed into eyes, rinse with water. It should be ventilated during use.

Huachenyang Technology Co., Ltd. was established on June 2, 2008. The company has 3 major business segments. The business operation center is located in Yifang Center, Baoan District, Shenzhen, the R&D experiment center is located in Nanshan District, Shenzhen, and the manufacturing center is located in Baoan, Shenzhen. Xinqiao Street, District, the company covers an area of ​​15,000 square meters, with a registered capital of 50 million yuan and more than 300 employees. A national high-tech enterprise integrating R&D, production, and sales of medical device products.

Huachenyang Technology is committed to biological sample collection and preservation of medical products. It is a well-known professional manufacturer of medical sampling and preservation products. The company has 52 patents, of which 30 patents have been transformed into the market. Products are mainly used in genetic testing, biopharmaceuticals, large-scale A-level hospitals, entry-exit inspection and quarantine, diagnostic reagents, CDC, public security criminal investigation, forensic identification, and other medical institutions. The company has successively established good cooperative relations with more than 30 domestic universities, more than 50 national scientific research institutions, more than 200 tertiary hospitals, more than 600 disease prevention and control centers, and more than 1,000 third-party medical testing institutions for genetic testing.

The company's independent research and development products include disposable virus sampling tubes, disposable sampling swabs, medical cotton swabs, cell preservation solutions, saliva collection devices, transport medium, disposable samplers, disposable sampler, sample preservation solution, Nucleic acid extraction reagents, genetic testing reagents, and other products. The company's products have obtained China NMPA, EU CE, US FDA EUA, SGS, TUV, TGA, and ISO13485 certification qualifications, and have the exclusive right to use registered trademarks and sales rights for multinational products. The products are exported to Europe, America, Asia, the Middle East, and other 120 countries. It has won unanimous praise and recognition from customers.

Shenzhen Huachenyang Technology Co., Ltd. will adhere to the tenet of "inheriting innovation, winning with science and technology", adhering to the corporate culture of "quality first, truth-seeking and innovation, integrity and law-abiding, cooperation and win-win", and strive to create China's high-end biological sample collection and preservation medical products, casting The industry benchmark for microbial sample collection and preservation products!