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Disposable Medical Dna Purification Kit PCR Detection Swab Kit
Huachenyang Medical Nucleic Acid Purification Kit PCR Detection Swab Kit Nucleic Acid Purification Kit
Product description:
This kit uses magnetic beads that can specifically bind DNA and a
unique buffer system. The silica-based magnetic beads and reagents
are used in combination. During the mixing process, the large
surface area of the magnetic beads and the sufficient connection
of the target make it have superior performance. Binding and
washing efficiency with high efficiency and specificity. It can
maximize the removal of impurity proteins and other organic
compounds in cells. The extracted DNA fragments are large, and the
purity and quality are stable and reliable.
Features:
Using silicon magnetic beads and buffer system with unique
separation function, it can quickly, highly sensitively and
efficiently extract high-purity nucleic acid from samples, and the
extracted and purified nucleic acid can be used for PCR, RT-PCR,
digestion, reverse transcription, and Southern Various common
downstream experiments such as blot, applicable specimen types:
whole blood, tissue homogenate, swab, serum, plasma,
bronchoalveolar lavage fluid, etc. Applicable extraction
instruments: most of the automatic nucleic acid extraction
instruments on the market
benefit:
1. Magnetic bead-based technology isolates genomic DNA without the
need for hazardous chemicals, centrifugation or vacuum manifolds,
phenol and ethanol precipitation.
2. Fast and efficient purification of genomic DNA from human buccal
swabs in less than 15 minutes after sample preparation and lysis.
3. Simple cleavage with proteinase K without any mechanical
cleavage.
4. Minimal RNA contamination.
5. Purified genomic DNA exhibits improved downstream performance in
applications such as PCR.
6. A kit is included for automated processing of large numbers of
samples in 96-well plates using a liquid handling robot.
model | CY-F006-11 (100 preps-cells) |
Reagent bottle size/volume1 | 60mL |
Reagent bottle size/volume2 | 60mL |
Reagent bottle size/volume3 | 60mL |
Reagent bottle size/volume4 | 125mL |
Reagent bottle size/volume5 | 15mL |
Product Instructions:
The magnetic bead material specifically adsorbs and dissociates
from nucleic acid under different salt concentrations and acid-base
conditions, separates nucleic acid and other components in the
lysed cells by the transfer magnetic bead method, and then obtains
the magnetic bead-free extract by washing and elution- -- Nucleic
acids.
The first step - lysis, use lysis solution to destroy the cellular
structure of animal and plant cells, so that the DNA is fully
released
The second step—binding, adding magnetic bead binding solution to
specifically adsorb DNA, so that DNA can be fully combined with
magnetic beads;
The third step------washing, using detergent, after multiple
washings, fully remove other residual impurities;
The fourth step - elution, use the eluent to dissociate the
magnetic beads and DNA to obtain a higher-quality DNA solution.
Precaution:
1. This product is only used for in vitro diagnosis.
2. The storage environment and extraction steps should be carried
out in strict accordance with the instructions in the instruction
manual.
3. If it is found that the amount is too small during the
extraction process, the sample size can be appropriately increased
or the number of extractions can be increased.
4. The extracted DNA must be fresh and tested in time.
Note: This transfer medium is intended for in vitro diagnostics and is not intended for internal or external use in humans or animals. If swallowed, it may cause a serious accident; it is irritating to eyes and skin. If splashed into eyes, rinse with water. It should be ventilated during use.