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SARS-COV-2 RT-qPCR Assay (Triple Fluorescence RT-PCR Method), Fluorescence RT-PCR
The SARS-COV-2 RT qPCR Assay is a real time (rt) reverse
transcriptase (RT) polymerase chain reaction (PCR) test intended
for the qualitative detection of ORF1ab and N genes of the SARS CoV
2 in nasopharyngeal ( NP) swab, oropharyngeal (OP) swab and sputum
specimen collected by a healthcare worker, from patients suspected
of COVID-19 by their health care provider.
High Accuracy
Limit of Detection: 200 copies/mL
Sensitivity: 100 %
Specificity: 99.1%
Accuracy: 99.3%
Convenient Operation
Triplex-detections in a single tube (ORF1ab & N genes, human RNAse P (RNP) gene)
Primer and probe premixed liquid form
Reliable
Anti-pollution system: UDG
Internal control: Human RNaseP (RNP) gene
Positive control: Pseudovirus
Fast
Result in about 1 hour
APPLICABLE INSTRUMENTS:
Real-time PCR instrument with FAM, TEXAS RED/ROX and HEX/VIC channels, such as ABI7500, ABI Q3, ABI Q6, Bio Rad CFX96.
Components | Amount SO tests | Amount 100 tests | Ingredient | Cap Color |
Reaction Mix | 675|ile2 tubes | 9OOMle3tubr$ | Orf lab, N genes and Human RNaseP (RNP) gene primers and pcob«$« Buffer, deoxy* ribonucl«o$ld« triphosphate (dNTPs) | Red |
Enzyme Mix Negative Control Package insert | 200^1,1 tube | 400yleltube | RNMe Inhibrtor, UDG・ Reverse Tnn$cripu$e, Yaq ONA polymerase RAN Pseudovirus Containing Target Gene (Orflab, N and Humin RN«$oP
(RNP) gerw ) / | Blue |
ItUD# 125pleltub« 1 | ZM411 Ituoe | Yellow |